| Scottish Premiership 1 |
| Sat 29 Aug 2009 | Ayr - Heriot's | (B) 37-21 |
|
| Sat 05 Sept 2009 | Watsonians - Ayr | 16-22 (B) |
|
| Sat 12 Sept 2009 | Ayr - Boroughmuir | (B) 31-13 |
|
| Sat 19 Sept 2009 | Edinburgh Academicals - Ayr | 18-25 |
|
| Sat 26 Sept 2009 | Ayr - Glasgow Hawks | (B) 32-25 |
|
| Sat 03 Oct 2009 | Dundee HSFP - Ayr | 24-22 (B) |
|
| Sat 10 Oct 2009 | Ayr - Melrose | 34-15 |
|
| Sat 17 Oct 2009 | Stew Mel Lions - Ayr | 13-31 (B) |
|
| Sat 24 Oct 2009 | Ayr - Selkirk | (B) 47-17 |
|
| Sat 31 Oct 2009 | West of Scotland - Ayr | 26-34 (B) |
|
| Sat 07 Nov 2009 | Ayr - Currie | 21-7 |
|
| Sat 14 Nov 2009 | Ayr - Watsonians | (B) 26-5 |
|
| Sun 29 Nov 2009 | Heriot's - Ayr | |
|
| Sat 05 Dec 2009 | Boroughmuir - Ayr | 6-13 |
|
| Sat 12 Dec 2009 | Ayr - Edinburgh Academicals | (B) 53-20 |
|
| Sat 19 Dec 2009 | Glasgow Hawks - Ayr | Reported |
|
| Sat 16 Jan 2010 | Melrose - Ayr | Reported |
|
| Sat 23 Jan 2010 | Ayr - Stew Mel Lions | (B) 66-15 |
|
| Sat 30 Jan 2010 | Selkirk - Ayr | Reported |
|
| Sat 20 Feb 2010 | Ayr - West of Scotland | Reported |
|
| Sat 06 Mar 2010 | Currie - Ayr | Reported |
|
| Sat 27 Mar 2010 | Ayr - Dundee HSFP | |
|
