| Guinness Premiership |
| Sat 06 Sept 2008 | Saracens - Harlequins | 21-24 |
|
| Sat 13 Sept 2008 | Harlequins - Bristol | (B) 31-13 |
|
| Sat 20 Sept 2008 | Gloucester - Harlequins | 24-20 (B) |
|
| Sat 27 Sept 2008 | Harlequins - London Irish | (B) 27-28 |
|
| Thu 02 Oct 2008 | Worcester - Harlequins | 23-30 (B)  |
|
| Sun 16 Nov 2008 | Harlequins - Wasps | 32-10 |
|
| Sat 22 Nov 2008 | Leicester - Harlequins | 27-14 |
|
| Sun 30 Nov 2008 | Harlequins - Bath | 21-14 |
|
| Sat 20 Dec 2008 | Northampton - Harlequins | |
|
| Sat 27 Dec 2008 | Harlequins - Leicester | |
|
| Sun 04 Jan 2009 | Wasps - Harlequins | |
|
| Sat 10 Jan 2009 | Harlequins - Worcester | |
|
| Sat 14 Feb 2009 | London Irish - Harlequins | |
|
| Sat 21 Feb 2009 | Harlequins - Gloucester | |
|
| Sun 01 Mar 2009 | Bristol - Harlequins | |
|
| Sat 07 Mar 2009 | Harlequins - Saracens | |
|
| Sun 15 Mar 2009 | Newcastle - Harlequins | |
|
| Sun 22 Mar 2009 | Harlequins - Sale | |
|
| Sat 28 Mar 2009 | Harlequins - Northampton | |
|
| Sat 04 Apr 2009 | Bath - Harlequins | |
|
| Fri 17 Apr 2009 | Sale - Harlequins | |
|
| Sat 25 Apr 2009 | Harlequins - Newcastle | |
|
